Contact an Account ManagerAn efficient hydrophilic interaction liquid chromatography separation of 7 phospholipid classes based on a diol column
Summary
Type: HPLC
Application #: 125800
Detection:
Conditions: Eluent A: 53 mmol/L formic acid in acetonitrile, pH 4.0–4.5
Eluent B: 60 mmol/L ammonium formate and 53 mmol/L formic acid in water, pH 3.64
Gradient: 90 % A for 2 min, 90-70 % B in 13 min, 70 % A for 2 min, 70-90 % A in 0.5 min, 90 % A for 12.5 min
Flow rate: 500 µL/min
Temperature: 50 °C
Autosampler tray temp. 15 °C
Injection volume: 10 µL
Authors: Zhu, C. et al.
Source: J. Chromatogr. A 1220 (2012), 26–34
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